Journal: Analytical and Bioanalytical Chemistry

Article Title: Measurement of the alcohol biomarker phosphatidylethanol (PEth) in dried blood spots and venous blood—importance of inhibition of post-sampling formation from ethanol

doi: 10.1007/s00216-021-03211-z

Figure Lengend Snippet: Formation of phosphatidylethanol (PEth 16:0/18:1) in two human blank whole blood samples fortified with 2 g/L ethanol during drying on a Capitainer qDBS microsampling device at room temperature

Article Snippet: Fig. 3 Box-and-whisker plots showing the formation of PEth 16:0/18:1 in human blank whole blood samples from 20 individuals fortified with 2 g/L ethanol and applied on filter paper (Whatman 903 Protein Saver card) or on three commercial devices for dried blood microsampling (HemaXis DB 10, 10 μL Mitra Clamshell, standard Capitainer qDBS, and qDBS devices fortified with 25 μg NaVO per filter disc).

Techniques:

Journal: Analytical and Bioanalytical Chemistry

Article Title: Measurement of the alcohol biomarker phosphatidylethanol (PEth) in dried blood spots and venous blood—importance of inhibition of post-sampling formation from ethanol

doi: 10.1007/s00216-021-03211-z

Figure Lengend Snippet: Box-and-whisker plots showing the formation of PEth 16:0/18:1 in a human blank whole blood (WB) samples from 10 individuals fortified with 2 g/L ethanol and applied and dried on dismounted Capitainer qDBS discs, with or without addition of NaVO 3 (25 μg/disc), and b blank whole blood samples from 10 other individuals fortified with 2 g/L ethanol and added on standard qDBS microsampling devices or devices fortified with 25 μg NaVO 3 per filter disc. The drying time was 3 h at room temperature. The PEth results in the presence of PLD inhibitor were always below the lower quantification limit (< 0.01 μmol/L). Please note the difference in scale in panels a and b

Article Snippet: Fig. 3 Box-and-whisker plots showing the formation of PEth 16:0/18:1 in human blank whole blood samples from 20 individuals fortified with 2 g/L ethanol and applied on filter paper (Whatman 903 Protein Saver card) or on three commercial devices for dried blood microsampling (HemaXis DB 10, 10 μL Mitra Clamshell, standard Capitainer qDBS, and qDBS devices fortified with 25 μg NaVO per filter disc).

Techniques: Whisker Assay

Journal: Analytical and Bioanalytical Chemistry

Article Title: Measurement of the alcohol biomarker phosphatidylethanol (PEth) in dried blood spots and venous blood—importance of inhibition of post-sampling formation from ethanol

doi: 10.1007/s00216-021-03211-z

Figure Lengend Snippet: Box-and-whisker plots showing the formation of PEth 16:0/18:1 in human blank whole blood samples from 20 individuals fortified with 2 g/L ethanol and applied on filter paper (Whatman 903 Protein Saver card) or on three commercial devices for dried blood microsampling (HemaXis DB 10, 10 μL Mitra Clamshell, standard Capitainer qDBS, and qDBS devices fortified with 25 μg NaVO 3 per filter disc). The storage time was 48 h at room temperature. The highest PEth concentrations were formed in the qDBS device (range 0.83–2.02, median 1.2 μmol/L; N = 20 samples), followed by HemaXis (0.04–1.04, median 0.39 μmol/L; N = 13), and Mitra (0.02–0.12, median 0.06 μmol/L; N = 8). No PEth formation was observed (< 0.01 μmol/L) with the Protein Saver card, or with the qDBS device fortified with NaVO 3 . When the ethanol-spiked blood samples were left in the test tubes for 48 h at room temperature, PEth was formed in all of them (range 0.03–0.08, median 0.04 μmol/L PEth)

Article Snippet: Fig. 3 Box-and-whisker plots showing the formation of PEth 16:0/18:1 in human blank whole blood samples from 20 individuals fortified with 2 g/L ethanol and applied on filter paper (Whatman 903 Protein Saver card) or on three commercial devices for dried blood microsampling (HemaXis DB 10, 10 μL Mitra Clamshell, standard Capitainer qDBS, and qDBS devices fortified with 25 μg NaVO per filter disc).

Techniques: Whisker Assay